View Article |
Isolation methods and culture conditions of human umbilical vein endothelial cells from Malaysian women
Raja Nor Suhaila1, Sabreena Safuan2.
Human umbilical vein endothelial cell (HUVEC) isolated from umbilical cord is widely used as endothelial cell model.
However, HUVEC has been characteristically hard to maintain and showed molecular heterogeneity depending on the
umbilical cord donors. Commercial HUVEC is commonly derived from European and Caucasian population which have
different molecular characteristics from Asian women. This study aimed to optimize the isolation and culture condition of
HUVEC using combinations of growth factors and extracellular matrix components so that the isolated HUVEC will purely
represent the population under study. Umbilical cords were obtained from women post-labour. Different incubation times
and digestive enzymes were used during endothelial cells isolation process. The culture conditions were optimized based
on the coating materials and the media supplements. The results showed that 0.1% collagenase for 40 min incubation
was the optimal isolation condition of HUVEC. HUVEC grown in 0.2% gelatin coated plate with 10% heat-inactivated
fetal calf serum showed higher proliferative capacity and reduced cell death compared to other conditions (p<0.05). The
results generated from this study provide a basic protocol of HUVEC isolation and culture conditions in order to generate
working endothelial cell populations purely represent the Malaysian population.
Affiliation:
- Universiti Sains Malaysia, Malaysia
- Universiti Sains Malaysia, Malaysia
Toggle translation
|
|
Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
|
6 |
Immediacy Index
|
0.000 |
Rank |
0 |
Indexed by |
Web of Science (SCIE - Science Citation Index Expanded) |
Impact Factor
|
JCR (1.009) |
Rank |
Q4 (Multidisciplinary Sciences) |
Additional Information |
JCI (0.15) |
Indexed by |
Scopus 2020 |
Impact Factor
|
CiteScore (1.4) |
Rank |
Q2 (Multidisciplinary) |
Additional Information |
SJR (0.251) |
|
|
|