The activated sludge system is an effective biological treatment applied widely in both industrial and municipal wastewater treatment facilities worldwide. Yet, the big issue arising from this process is the huge amount of sludge production at the end of the process. Thus, this study was aimed at studying the sludge disintegration and solubilisation efficiency of activated sludge from rubber glove processing industry using ozonation. The ozonation was conducted in batch process, and the sample was analyzed after 10, 20, 30, 40, 50 and 60 minutes of contact time. It was found that ozone was able to cause the bacteria in the activated sludge to undergo lysis, which solubilised the organic matter. The settling property was improved with the SVI reduced from 103 to 79 mL/g. Ozonation at 40 minute with ozone consumption of 22.7 mgO3/gTSS was able to reduce SS, FS, and VSS to 16.6%, 19%, and 14%, respectively. The SCOD solubilisation was achieved at a maximum of 71.8%, while the nitrogenous compound of NH3-N was at 34% due to the release of organic matter from EPS and sludge cells after 60 minutes of treatment. A graph was plotted to evaluate the correlation between sludge disintegration and solubilisation, which showed a positive correlation (R2=0.970). Thus, ozonation was found to be an efficient method to disintegrate and solubilise the activated sludge from rubber processing industry.