The field of nanotechnology is advancing rapidly due to its extensive applications in distinct fields of science and technology. Several methods have been used for the production of nanoparticles due to their immense functions in various fields. The limitations of the traditional methods and their toxic nature of the chemicals used during the synthesis or in their application hinder their usage in the biomedical field. This led to the development of the green synthesis of nanoparticles. Researchers have focused on developing simple, cost-effective, clean, non-toxic and eco-friendly procedures for synthesis of nanoparticles. Various biological agents like bacteria, fungi, plant extracts, etc. are used for the green synthesis of metal nanoparticles due to their biocompatibility. The dissolved metals ions are reduced into nano-metals by bio-agent in the green process. The manners and protocols of the green synthesis of gold nanoparticles are presented in this review. Recent literature on green synthesis of noble gold nanoparticles with their various applications in biomedical, antifungal/antibacterial, drug delivery, sensors and photocatalytic have also been discussed.