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The osteoprotective effect of azadirachta excelsa leaves extract on bone of streptozotocin-induced diabetic rats
Nur Syimal`ain Azmi1, Nooraain Hashim2, Nurdiana Samsulrizal3, Noor Syaffinaz Noor Mohamad Zin4.
Insulin signalling in bone favours whole-body glucose homeostasis by
activating osteocalcin, which is important for bone remodeling. However,
diabetes causes deficient production of insulin which consequently affects
the osteocalcin and bone turnover marker. The aim of this study was to
explore the potential of A. excelsa to improve insulin and osteocalcin
secretion, resulting in improved bone histomorphometric and bone turnover
marker in STZ- induced diabetic rats. The experimental rats were divided into
normal control (NC), diabetic control (DC), Metformin-treated diabetic
(DMET) (positive control) and A. excelsa-treated diabetic (DAE) rats with the
treatment period of eight weeks. After the treatment, the femoral bones
were removed and bone morphometrical parameters were defined using
Micro-CT scan. The bones were analysed for mineral density (BMD) and
trabecular parameters. The bones samples were decalcified for histological
preparation and images of the was hematoxylin and eosin (H&E) stained
bones were captured and analysed. The concentrations of serum insulin,
osteocalcin and bone turnover marker were evaluated using specific ELISA
kits. The study showed that A. excelsa caused a significant increase in insulin
and osteocalcin levels. A. excelsa also represents ameliorative effects on
trabecular bone of the diabetic rat. The data also demonstrated that with
A. excelsa treatment, it moderately restored the balance between bone
formation and bone resorption markers. These data confirmed that A.
excelsa extract could attenuate the STZ-induced bone loss and reverses the
deterioration of bone microarchitecture in diabetic rats. This finding
indicates the osteoprotective effects presence in the A. excelsa extract.
Affiliation:
- Universiti Teknologi MARA, Malaysia
- Universiti Teknologi MARA, Malaysia
- Universiti Teknologi MARA, Malaysia
- Universiti Teknologi MARA, Malaysia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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6 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (1.4) |
Rank |
Q3 (Engineering (all)) |
Additional Information |
SJR (0.191) |
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