This article presents an exploration and investigation of the eye movement features to track the eye-mind relationship through eye tracking device during video clips of positive emotional stimulation. The relationship of eyes gazing behavior with human mind is a challenging topic. Many parameters of the eyes gazing behavior is yet to be explored namely pupil size and pupil dilation. Since pupil size is one of the indicators of the brain’s activity studies on eye-mind relationship can be beneficial and added value in learning human behavior. Prior studies have suggested that the cognitive processing and affective information affect the size of pupils in humans. Significantly, this study focus on the behavior of positive emotion by using eye tracking technique to observe the eye-mind relation. It is hypothesized from this observation that the visual attention of the gazing behavior will affect the pupil dilation and will further provide evidence of the human mind triggered emotion. Ten subjects’ pupil responses were measured while watching interesting and amusing emotional clips. The results showed that the fixation duration and pupil dilation significantly different between each video stimulation. These results suggest that the measurement of eye fixation is a potential computer input for detecting emotional state.