Agriculture activity in Cameron Highland is one of the sources of river water pollution. This study evaluates the phys- icochemical properties and heavy metals contamination (Cu, Cd, Zn, Cr, Pb) in Bertam River. The results indicate the river water pH was slightly acidic. Based on the Malaysia National Drinking Water Quality Standard (NDWQS), the river water is not suitable for drinking as it has exceeded the safe value for turbidity, chemical oxygen demand COD and lead (Pb). Spatial distribution of heavy metals showed that Cr, Cu and Pb were concentrated at the downstream area.