Introduction: Temporomandibular joint disorders (TMD) often characterized by craniofacial pain, include joints, masticatory muscles. The main cause is because of tooth loss and bad habits. The age of patients with TMD is from the age of 20 to 40 years. The DC / TMD protocol is a TMD examination protocol that is highly recommended for use as a clinical and research protocol. The objective of this study was to determine the relationship of tooth loss with TMD using the DC / TMD protocol. Method: This research is a descriptive analytic study with cross-sectional design. The sample consisted of 209 FKG YARSI academic students taken by total sampling. TMD assessment uses the DC / TMD protocol. Results: The results of the ken-tau test showed that there was a significant relationship between tooth loss and TMD, namely p-value = 0.024 (<0.05). Conclusion: There is a significant relationship between tooth loss and temporomandibular disorder according to DC / TMD. The most common description of temporomandibular disorder diagnosis in YARSI University Faculty of Dentistry academic students is disc displacement with reduction.