Introduction: The diabetic condition may delay collagen formation of the wound due to prolonged inflammation. The seeds of Bixa orellana contains some active substances that may accelerate collagen formation during wound healing. The objective of this study was to determine the effect of topical application of B. orellana seed ethano-lic-extract-hydrogel-patch on the density of collagen fiber of rat diabetic wound healing model. Method: Thirty male Wistar rats (8-12 weeks-old, 150-200grams) were induced into diabetic state (fasting blood sugar level >150mg/dL) via injection with streptozotocin (40mg/kg BW, ip). Following confirmation of the blood sugar level, the animals were then divided into treatment and control group (n=5 rats/group). The back skin was wounded using punch-biop-sy-instrument. The hydrogel patch with or without the extract was applied on the oral and skin wounds once a day during the treatment. The subjects were sacrificed on day 5, 7, and 14, histologically processed, and Mallory-stained. The density of collagen fiber was scored from five view fields under microscope. The data were analyzed using Kru-skal-Wallis statistical test. Results: The study showed that the extract significantly accelerated the formation of the collagen fiber on the skin of the treated group compared to the control group (p<0.05). Conclusion: The application of B. orellana seed extract-hydrogel-patch affected collagen fiber density of the skin, and, therefore, may accelerate wound healing on a diabetic model.