Introduction: Chitosan is known as biopolymers that offer advantages such as biocompatibility, biodegradability, lower antigenicity, antibacterial and renewability. It can be modified with collagen as a composite dressing for wound healing. The aim of this study was to determine the activity of chitosan as an antibacterial in chitosan-colla-gen composite dressing. Methods: This experimental study was divided into group K0, K1, K2, and K3. K0 as control group used pure chitosan, K1 group made by collagen 25% and chitosan 75%, K2 group made by collagen 50% and chitosan 50%, K3 group made by collagen 75% and chitosan 25%. Chitosan powder was dissolved in acetic acid 2%, then mixed with acid soluble collagen and dried at room temperature. Each of the composite then were growth in nutrient agar plate to count total bacteria. Results: This study showed that no bacteria were recovered on K0, K1, K2 plates, but K3 had total plate count of 42 CFU and statistically showed there was discrepancy for each group with p-value was 0,002 (p<0,05). Conclusion: Chitosan had the effect as antibacterial properties based on its concentrations in composite dressing.