Introduction: Mesenchymal stem cells taken from adipose tissue have a more accessible access advantage, their retrieval techniques are painless and have multipotent properties which can differentiate osteogenically. Therefore, they have bone regeneration ability in vitro and in vivo studies. The use of allogeneic adipose-derived mesen-chymal stem cells (ADMSCs) can regenerate damaged tissue with high-quality without fibrous tissue formation, minimal donor morbidity compared to autografts, and hypo-immunogenic responsiveness. The purpose of this study was to analyze the acceleration of the administration of allogeneic ADMSCs from rat and demineralized dentin matrix (DDM) scaffolds to regenerate alveolar bone resorption in the periodontitis model. Methods: Twenty-five Wistar rats were divided into three groups: control, DDM scaffold group and ADMSCs-DDM composite group. Alveolar bone resorption was induced by injection of LPS P.gingivalis for six weeks in the right mandibular molars. Transplantation was done in periodontal pockets for 7 & 14 days. The parameters were assessed by using scanning electron microscope (SEM), micro-computed tomography (μ-CT), expression collagen type I by immunohistochem-istry (IHC) staining and trabecular area and osteoblast with hematoxylin staining (HE). Statistical Analysis was done by using Mann Whitney (p <0.05). Results: There was a significant difference in the expression of collagen type I on the 7th and14th days. Where the expression on day 14 showed significant results (p <0.05) in the ADMSC- DDM composite group compared to DDM scaffold and controls groups. The trabecular area and osteoblast cell count of ADMSC-DDM composite on day 14 was significantly higher than the DDM and control groups (p<0.05). Conclu-sion: This study concludes that the composite of ADMSCs-DDM on periodontal pockets of Wistar rats has the ability in alveolar bone regeneration.