Introduction:Thrush or ulceration is inflammation that occurs in the oral mucosa including the gingiva, cheek, pal-ate, lip tongue and mouth floor. Wound healing process is strongly influenced by fibroblast cells. Nanogel Jarak Cina latex extract contains alkanoid, latexonin, tannin and flavonoid as antioxidants, antibacterial, anti-inflammatory, and increases collagen type 1 that it can accelerate wound healing of ulceration. The aim of this study was to determine the differences in the number of fibroblasts in healing traumatic ulcer Wistar rats (Rattus norvegicus) after adminis-tration of Jarak Cina latex extract (Jatropha multifida L). Method: experimental study, used the Randomized Post test only control group design. Every group were divided in four groups, where 3 groups treated with Jarak Cina latex extract and 1 group without treatment. On the fifth day mice were decaputated and tissue histology preparations were made to count the number of fibroblasts. Result: Homogeneity and normality tests show that the data is homo-geneous and normally distributed. The results showed the group P1 with a 1% concentration has the highest number of fibroblasts, followed by a group P2, P3 and the control group. Conclusion: Based on this study it can be concluded that there are differences in the number of fibroblast cells in Wistar rats (Rattus norvegicus) for the administration of Jarak Cina nanogel extract (Jatropha multifida L).