Introduction: Heat cured acrylic resins are often used as material from artificial teeth, especially removable denture bases. The existence of microcavities in acrylic becomes a place of attachment of food scraps that can increase the number of microorganisms in the oral cavity, one of which is Candida albicans. The use of dentures that are con-stantly and not clean can cause an increase in the number of Candida albicans colony growth is rapid, making it the main cause of infection in the oral mucosa called denture stomatitis. Maintenance of denture hygiene can be done in several ways, namely mechanically and beggars. Chemical cleaning is usually the most widely used because it is easier to clean dentures from microorganisms such as the number of Candida albicans colonies on the acrylic heat-cured denture base material. Betel leaf extract containing essential oils and the main component is phenol which has an antifungal effect. Method: The purpose of this study was to determine the effect of 10%, 15% betel leaf extract and polydent solution as a positive control with 1 hour and 6 hours immersion on the growth of Candida albicans colonies on a heat-cured acrylic resin plate which was purely experimental laboratory using the post-test method control group design only. Result: The results of statistical calculations with the Kruskal-Wallis Test revealed differ-ences between the betel leaf extract group and the control group with soaking time, followed by the Mann-Whitney test to determine differences in effectiveness between the 2 concentrations in the betel leaf extract group and the immersion time (p <0.05). Conclusion: From the results of the study it can be concluded that the betel leaf extract can kill the growth of Candida albicans colonies on a heat-cured acrylic resin plate with high concentration and length of soaking time.