Introduction:Over the last decades, caries management is moving from restorative method into regenerative by using autologous stem cell transplantation. It is important to gain high quality human postnatal stem cells from accessible resources. Stem Cell from Human Exfoliated Deciduous Teeth/SHED is stem cell source with extensive proliferation capacity and extracted primary teeth are easy to obtain. After being isolated, SHED is then growth in culture in initial cell density. In order to gain stem cell in abundant supply, initial density might effect cell growth. This study was aimed to analize effect of initial cell density in SHED proliferation capacity. Methods: The research was experimental laboratory with cross sectional design. The sample was obtained from dental pulp of primary ex-tracted teeth which matched the criteria. SHED was seeded in different initial cell density that is 500 cell/well, 1000 cell/well, 2000 cell/well and 4000 cell/well. Cell proliferation then being analized using MTT assay. Results: The average number of SHED seeded in initial cell density 2000 cell/well and 4000 cell/well showed the highest prolif-eration with no statistical difference. Conclusion: In this study, initial cell density 2000 cell/well showed optimal proliferation capacity of SHED. SHED is very accessible tissue resource and also potential in providing abundant cells for clinical application.