Introduction:The wound healing process can be accelerated by topical medication. Snail mucus is one of the tradi-tional medicine which commonly used by the community, because it has Acharin Sulfate and Glycosaminoglycan. This study aims to determine the effect of snail mucus topical application with concentrations 2,5%, 5% and 7,5% for post tooth extraction wound healing in guinea pigs. Method: Quasi-experimental research was conducted on 24 guinea pigs, which were divided into 4 groups. The first group was given topical application of Povidone Iodine 10% on H0, the other groups were given snail slime gel 2.5%, 5% and 7.5% on D+1 and D+2 after extraction of left incisors, respectively. Standard photo taking is done on D0, D+3, D+5, D+7, D+10 with a DSLR camera. Wound area measurements using the 2016 Skech-up program. Inflammation scores were assessed by drawing marginal lines in the wound tissue and then dividing it into 8 sections. The section with inflammatory was given 1 score. Data were analyzed by Kruskall Wallis and Mann Whitney at a significance level of 5%. Result: The results showed that on each day of observation each group experienced a reduction in wound area and inflammation score. The difference in wound area was 7.5% for the control group and 2.5% was significant for each day of observation. The difference in wound area in the 5% group and the 7.5% group was significant in D+3, D+5 and D+7 observations, but in D+10 it was not significant. The difference in inflammation score of the 7.5% group and the control group was significant at D+7 and D+10. Conclusion: It was concluded that the application of 5% and 7.5% snail slime gel could accelerate wound healing after guinea pig’s tooth extraction with the same effect on the 10th day observation.