Introduction:Diabetes mellitus can cause delayed wound healing include in oral cavity because of fibronectin for-mation decreases. Propolis is one of the herbal medicines commonly used as an alternative medicine for oral ulcer. The study explored the role of propolis extract gel application from beekeeping Malang Lawang Regency on the expression of fibronectin in the healing process on diabetic Rattus novergicus with traumatic oral ulcer. Methods:The study was conducted on 27 male Rattus novergicus divided into nine groups. Sixth groups were induced by 50 mg/kg of Streptozotocin to provoke hyperglycemia for positive control and treatment groups. Three groups without Streptozotocin for negative control. All of the groups suffering traumatic ulcer on their lower lip mucosa. The control groups treated with HPMC 5% gel and propolis extract gel was applied to treatment groups. The fibronectin expres-sion was observed on days 3, 5, and 7. Rattus novergicus sacrificed and the lower lip labial mucosa tissue has been taken for the histopathological anatomy preparation using immunohistochemical examination with monoclonal an-tibodies anti-fibronectin. Results: This study revealed the expression of fibronectin increased in the treatment group rather than positive controls resembling expressions in normal conditions. Analysis of variance showed significant differences (p<0.05) of fibronectin expression between the treatment and positive control groups on days 5 and 7. Conclusion: Propolis extract gel increase the expression of fibronectin during the healing process of traumatic ulcers on the oral mucosa of diabetic Rattus novergicus.