Introduction:Carica papaya variety California is widely distributed in Indonesia. The pulp is used for daily con-sumption, but the seed and peel are usually discarded. Even though the seed and peel of Carica papaya are often discarded, they may have a potential as a herbal medicine. Previous studies have shown that Carica papaya’s seeds and leaves can inhibit the growth of Candida Albicans. Carica papaya variety California contains anti-fungal proper-ties such as saponin, alkaloids, flavonoids, steroids and tripenoids, but the anti-fungal effects of Carica papaya vari-ety California’s peel against Candida Albicans has not been established yet. Therefore, this study aims to determine the anti-fungal potential of the extract of Carica papaya variety California’s peel against Candida Albicans colony. Methods: The 2.5%, 5%, 10% and 20% concentration of Carica papaya variety California’s peel was prepared. Each concentration was tested with Candida Albicans culture in Sabouraud dextrose agar, using aquadest as negative control and Nystatin 1:100,000 IU as positive control. The minimum inhibitory concentration was evaluated by Kir-by-Bauer Disk Susceptibility Test. Results: No minimum inhibitory concentration was found in 2.5% concentration, whereas low minimum inhibitory concentration was found in 5%, 10% and 20% concentration of Carica papayavariety California’s peel extract. Conclusion: Extract of Carica papaya variety California’s peel with a minimum con-centration of 5% might be used as an anti-fungal agent against Candida Albicansin vitro. Futher investigation need to be performed to evaluate the potential of Carica papaya variety California’s peel as a herbal medicine in vivo.