Introduction: Patients with diabetes mellitus (DM) tend to experience decreased levels of Epidermal Growth Factor (EGF) that can slow wound healing process, including in the oral cavity. This study was to determine the effects of propolis extract gel on EGF expression in the traumatic oral ulcers of Wistar rats suffered with diabetes mellitus (DM). Methods: The study was conducted on 36 male Wistar rats (Rattus novergicus) induced intraperitoneally by 50 mg/kg of Streptozotocin to provoke hyperglicemia, and a traumatic ulcer on their lower lip mucosa. The Wistar rats were divided into twelve groups; three for each positive control, negative control and treatment groups. Each control and treatment group consisted of three rats. The control groups were treated with HPMC 5% gel and propolis extract gel was applied topically on treatment groups. The expression of EGF was observed on day 3, 5, 7, and 9. Furthermore, the rats were sacrificed and the lower lip labial mucosa tissue of rats has been dissected for the immunohistochem-ical examination with monoclonal antibodies anti-EGF. Results: This study revealed that there was a the expression of EGF was increased in the treatment groups rather than positive controls. Analysis of Variance showed significant differences (p≤0.05) of EGF expression between the treatment groups and positive control groups. Conclusions: The use of propolis extract gel can increase the expression of EGF that can activate cell proliferation which plays an im-portant role in the process of wound healing.