Introduction: When a wound occurs, several growth factors are released together with inflammatory mediators, such as epidermal growth factor (EGF). Pangas catfish gelatin contains abundant amino acids that play an important role in wound healing process. This study aimed to investigate the effect of pangas catfish gelatin on the level of EGF expression in dental socket following tooth extraction of albino rats. Methods: Study samples were divided into three control groups (K) and three treatment groups (P) evaluated on the third, fifth and seventh days after tooth extraction. Expression of EGF in dental socket was assessed using immunohistochemical method. Results: The mean levels of EGF expression in post-tooth extraction wounds of the control groups (K) on the third (K1), fifth (K2) and seventh (K3) days were 214.5, 283.25 and 370.45, respectively, meanwhile those of the treatment groups (P) on the third (P1), fifth (P2) and seventh (P3) days were 310.35, 457.7 and 512.4, respectively. Conclusion: Administration of pangas catfish gelatin increases the mean level of EGF expression from the third day to the seventh day. Accordingly, application of pangas catfish gelatin may be considered for the wound healing treatment.