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Altered mucosal-associated invariant T cells phenotype in children with newly diagnosed type 1 diabetes but not in autoantibody-positive at-risk children
Ahmad Mahfuz Gazali1, Kirsti Näntö-Salonen2, Reeta Rintamäki3, Jussi Pihlajamäki4, Mikael Knip5, Riitta Veijola6, Jorma Toppari7, Jorma Ilonen8, Tuure Kinnunen9.
Introduction: Mucosal-associated invariant T (MAIT) cells are unconventional T cells, enriched in the gut. They ex-press an invariant T-cell receptor and recognize riboflavin metabolites from bacteria presented by MHC-Ib-related protein 1 (MR1) molecules. Alterations in gut microbiota have been reported in patients with type 1 diabetes (T1D), even before the onset of the disease. These changes can potentially alter the frequency or phenotype of circulating MAIT cells. Methods: We characterized peripheral blood MAIT cells in a cohort of 51 children with newly diag-nosed T1D, 27 at-risk children positive for multiple autoantibodies (AAb+) and 113 age-matched healthy children. Using multi-colour flow cytometry, we analysed the frequency, surface phenotype and cytokine production of MAIT cells. In addition, we characterized the frequency and surface phenotype of blood MAIT cells in 26 patients with long-standing T1D and 25 age-matched healthy controls. Results: No significant differences in MAIT cell frequency were observed between the study groups. Further phenotyping revealed that the expression of CD8, CD27, CCR5 and β7 integrin on MAIT cells was lower in children with newly diagnosed T1D compared to AAb+ and healthy chil-dren. The frequency of MAIT cells producing IFN-γ was also lower in children with newly diagnosed T1D, but the frequencies of IL-17A- and IL-4-secreting MAIT cells were similar in the study groups. Finally, the capacity of MAIT cells to be activated in vitro by E.coli bacteria through MR1 was comparable between the study groups. However, none of these changes was observed in adult patients with long-standing T1D. In contrast, a decreased frequency of MAIT cells and increased CD25 expression was observed in adult T1D patients with a short duration after diagnosis. Conclusion: There are subtle changes in the circulating MAIT compartment in patients with T1D at the onset of the disease as well as after clinical diagnosis, but not in AAb+ at-risk subjects including progression to clinical disease. Consequently, the alterations in blood MAIT cells are likely associated with the clinical manifestation of the disease rather than being features of earlier T1D autoimmunity.
Affiliation:
- University of Eastern Finland, Finland
- Turku University Hospital, Finland
- University of Eastern Finland, Finland
- University of Eastern Finland, Finland
- Tampere University Hospital, Finland
- Oulu University Hospital, Finland
- Turku University Hospit, Finland
- Turku University Hospit, Finland
- University of Eastern Finland, Finland
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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3 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
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