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Lactoferrin potential to increase fibroblast cell number and collagen in the Wistar rat wound healing process
Istiati1, Retno Pudji Rahayu2, Mochamad Aldy Sudarminto3.
Introduction: Wounds can be defined as damage or separation of the skin, mucous membrane or tissue caused by the influence of physical, mechanical and biological injury. The healing process is divided into 3 stages, namely: inflammation, proliferation, and remodelling. Lactoferrin is a glycoprotein with iron-binding properties. Lactoferrin is an 80-kDa iron-binding glycoprotein within the transferrin family. Lactoferrin has various biological functions, in-cluding roles in iron metabolism, cell proliferation and differentiation, antibacterial, antiviral and antiparasitic activ-ity. Lactoferrin will stimulate the work of macrophages which will produce various pro-inflammatory cytokines and anti-inflammatory cytokines, as well as various growth factors. Lactoferrin can trigger a decrease in pro-inflammatory cytokine processes but also activate anti-inflammatory cytokines, namely Transforming Growth Factor Beta (TGF-β), Platelet-Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF). fibroblast and collagen coir density. The aim of this study is to prove that lactoferrin can increase the number of fibroblast cells and collagen husks in the wounds of Wistar rats. Methods: This research is a laboratory experimental type with a post-test only control group research design. The sample consisted of 24 Wistar rats divided into four groups, namely the control group (K), and the treatment group treated with lactoferrin 50% (A1) for 3 days, the treatment group treated with lactoferrin 70% (A2) for 3 days and the group the treatment was treated with lactoferrin 90% (A3) for 3 days. The results of the study were obtained from histopathological examination, namely HE staining to observe the number of fibroblast cells and MT staining to observe the amount of collagen coir density. Results: There is an optimal increase in the number of fibroblasts and coir density of collagen by administration of Lactoferrin in the group of 70% (A2). Conclusion: Giving lactoferrin in a concentration of 70% can increase the number of fibroblast cells and collagen density in the wound healing process in Wistar rats.
Affiliation:
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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3 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
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