View Article |
Studying the ability of immunoglobulin Y anti-Porphyromonas gingivalis against periodontopathogen bacteria adherence
Sidarningsih1, Rini Devijanti Ridwan2, Indeswati Diyatri3, Tuti Kusumaningsih4, Nova Andriani Hepitaria5.
Introduction: Periodontitis is mainly caused by gram-negative bacteria. Porphyromonas gingivalis (P.gingivalis) is the main bacterium that causes chronic periodontitis and Aggregatibacter actinomycetemcomitans (A.actinomy-cetemcomitans) causes aggressive periodontitis. Egg yolk antibodies are an innovative technology that involve the formation of non-invasive polyclonal antibodies from egg yolk. IgY in the form of polyclonal antibodies is used in the form of passive immunization and these antibodies come from egg yolks, colostrum or concentrated cow’s milk. IgY antibodies have the same biological role as IgG antibodies in mammals, namely as the main immunoglobulin that provides defense from infectious agents. The aims of this research to prove the ability of egg yolk IgY in inhibiting the occurrence of periodontopathogen bacteria. Methods:P.gingivalis ATCC 33277 was cultured in MHA and incu-bated at 37°C for 48 hours using an anaerobic jar. The bacterial culture was centrifuged at 6000 rpm, at 4°C for 15 minutes then suspended in PBS containing 1% BSA. The bacterial content was made to 108/ml, IgY anti-P.gingivaliswas made to 7 concentrations, with each containing 50 μl IgY solution. Fifty μl enterocyte suspension was added to each concentration and shaken slowly in a shaking water bath at 37°C for 30 minutes. The bacterial suspension (108/ml) was added as much as 50 μl and incubated in a ‘shaking incubator’ for 30 minutes at 37°C. The samples were subsequently centrifuged at 1500 rpm, at 4°C for 3 minutes, smeared on a glass slide and stained with Gram stain-ing. The preparations were observed under a 1000x magnification microscope, and the number of bacteria attached to the enterocytes were counted for each observation of 100 enterocytes. Results: This study shows that IgY anti-P. gingivalis can significantly reduce the adherence index value of P.gingivalis, A.actinomycetemcomitans, and can reduce the adherence index value of F. nucleatum but not significantly. Conclusion: IgY anti P. gingivalis can inhibit P. gingivalis and A. actinomycetemcomitans adherence, but cannot inhibit F. nucleatum adherence.
Affiliation:
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
|
|
Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
|
3 |
Immediacy Index
|
0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
|
CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
|
|
|