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Correlation of susceptibility to antiseptics with biofilm-forming properties in acinetobacter baumannii as a pathogen of surgical infection
Vasyl Nahaichuk1, Oleksandr Nazarchuk2, Mariia Faustova3, Maiia Ananieva4, Oksana Turzhanska5.
Introduction: The purpose of this research was to study the biofilm-forming properties of clinical strains of A.bau- mannii, isolated from burn wounds in patients of ICU, and their sensitivity to antiseptics. Methods: 220 clinical strains of A. baumannii isolated from intensive care unit infected burn patients were the object of the study. Antisep- tic sensitivity of Acinetobacter spp. (decamethoxine, chlorhexidine, miramistin, povidone iodine) was investigated using double serial dilutions according to the standard procedure. The study of biofilm-forming properties of clinical Acinetobacter isolates was performed using the spectrophotometric technique by G.D. Christensen. In order to de- termine the relationship between the antiseptic sensitivity and biofilm-forming properties of A. baumannii strains, we determined the correlation coefficient (r-Pearson), the absolute value of which characterised the binding force. Results: Among 435 burn persons, who were involved in the investigated group, representatives of Acinetobacter spp. were found in 220 (50.6%), that has revealed the etiological significance of the opportunistic pathogens of Acinetobacter isolates in the development of infectious complications of burns in intensive care units. Clinical strains of A. baumannii have shown variable susceptible to decamethoxine, chlorhexidine, miramistin, povidone iodine and have been found to possess high biofilm-forming properties. The r-Pearson coefficient between sensitivity of A. baumannii to investigated antiseptics and biofilm-formation pointed out positive moderate and strong correlations. Conclusion: Biofilm-formation of Acinetobacter spp. is correlated with their susceptibility to chlorhexidine and povi- done iodine strongly. However, as a more powerful antimicrobial activity of antiseptic against A. baumannii was as weaker correlation had been established.
Affiliation:
- National Pirogov Memorial Medical University, Ukraine
- National Pirogov Memorial Medical University, Ukraine
- Ukrainian Medical Stomatological Academy, Ukraine
- Ukrainian Medical Stomatological Academy, Ukraine
- Vinnytsia Mykhailo Kotsiubynskyi State Pedagogical University, Ukraine
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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3 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
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