Formative assessment is an important tool to gauge the competence and performance of a medical student. This type of assessment enable the students to identify their strengths and weaknesses in order to improve their learning. The feedback given to students will motivate them to achieve their learning goals. The advancement of technology increases the usage of online-based assessment as it is more relevant and appealing especially to the younger generation. Online-based assessment such as Kahoot! offers immediate feedback and enhances the students’ critical thinking and problem solving skills in a fun environment. These skills are important to be assessed especially following Problem-based Learning (PBL) sessions. PBL is adapted in many medical schools worlwide. Due to the nature of extensive learning objectives in PBL, it is important to have a practical formative assessment like quizzes in Kahoot! platform to assess the students’ understanding. This study investigates the pre- clinical medical students’ perception about Kahoot! quiz challenge as a formative assessment tool following PBL sessions. Summary of work: Kahoot! quiz challenge was done following the completion of the second session of PBL in pre-clinical medical students. The quiz consisted questions related to the learning objectives of that particular PBL session from various subjects. At the end of the year, an online survey was carried out on the pre-clinical medical students on their perception of kahoot! Quiz challenge following PBL session. The modified validated questionnaire used has 12 items related to the students’ perception on Kahoot! quiz challenge. Each item consists of 5-point scale; ranging from 1 (strongly disagree) to 5 (strongly agree) in which the students must rate each item. The data were then analysed, descriptive statistics and Mann- Whitney test was performed. Summary of results: A total of 243 students participated in this survey with a majority of female respondents (76.1%). All the items in the survey has the median score of 4 in which the students agree that Kahoot! quiz challenge helped them to focus on the subjects, motivates them to learn, enhances their understanding, simplifies complex subject and facilitates learning. The students also agreed that Kahoot! quiz challenge is fun, more enganging and is effective in providing feedback. Conclusions: Kahoot! quiz challenge is useful as a formative assessment in PBL. It helped the students’ understanding and also motivated them to learn.