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Combination of blue laser exposure with UV-LED to improve antimicrobial effects on Staphylococcus aureus biofilm
Suryani Dyah Astuti1, Suhariningsih2, Asif Rasheed3, Inda Rahmawati4, Putri Suryaning Puspita5, Septia Kholimatussa’diah6, Alfian Pramudita Putra7, Pujiyanto8.
Introduction: Staphylococcus aureus biofilm is a major mediator of infection. The light based therapy is still not ad- equate to eradicate biofilm caused by Extracellular Polymeric Substances (EPS). The Ultra Violet (UV) irradiation has high energy and sufficient to penetrate a tissue. This study aims to investigate the effect of combination of UV LED irradiation and blue laser on the biofilm. Methods: The pure culture of Staphylococcus aureus ATCC 25923 approx- imated 108 CFU/mL or 1.0 McFarland Standard was used for this study. The biofilm sample was placed onto micro plate for 48 hours. The treatment group was divided into 3 groups, which were blue laser group, UV LED group and UV-Blue laser group. Results: The results showed that the highest biofilm reduction (80.57 ± 0.77) % was treated by blue laser irradiation for 4 minutes and UV irradiation for 20 s. Conclusion: Thus, the combination UV LED and Blue laser is the best choice to eradicate more biofilm.
Affiliation:
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Sepuluh November Institute of Technology, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
- Universitas Airlangga, Indonesia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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3 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
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