Introduction: Medical students often experience sleep deprivation due to a large academic load. Sleep insufficiency is one hypertension modifiable risk factors, yet its pathophysiological mechanism is still under-researched. This study aims to find out the sleep quality profiles of medical students in Malang-Indonesia and explore the effects of sleep insufficiency on systolic blood pressure and Nitric Oxide (NO). Methods: A total of 153 medical students completed the Pittsburgh Sleep Quality Index questionnaires. Forty students (40) participants were randomly selected into two groups to explore the comparison of NO concentration and the systolic blood pressure. The systolic blood pressure was measured with a sphygmomanometer. NO concentration was assessed with ELISA using the saliva sample. Results: Most of the medical students (89.54 %) had poor sleep quality with the average sleep time for 4 hours. The independent t-test showed significant differences in systolic blood pressure and NO concentration between two groups (p<0.05). Nitric oxide negatively influenced systolic blood pressure (p<0.05, R=-0.337). Conclusion: Medical students experienced poor sleep quality and sleep deprivation. Sleep insufficiency increases the systolic blood pressure. The increase of NO concentration may indicate the normal vascular endothelial response due to sleep loss in young adults.