Introduction: Physical inactivity has been considered as a pathological condition and additionally become a very important risk issue that plays role in inflicting fatalities. Plyometrics training is a form of exercise that can effectively reduce the fatalities prevalence caused by physical inactivity. It also increases the cognitive and affective ability. This study aims at assessing the effects of plyometrics training on short-term memory and mood states in Diponegoro Medical Faculty Students. Methods: A quasi-experimental study with 36 medical students (15-25 years old) as subjects. The subjects have been divided into two clusters: EP and C cluster. The EP cluster was given plyometrics training for six weeks and every week consists of two-session exercises and each session contains 5 movements. The C cluster was control group and didn’t perform plyometrics training. The subjects were chosen purposively and participated in two tests (scenery picture memory test and profile of mood states) before training and after six weeks of training. Paired t-test and independent t-test were used for statistical analysis. Results: There was a major distinction in scenery picture memory test score in the plyometrics training cluster compared to the control with p < 0,001. Mood measurement stated in the total mood disturbance score (TMD) also showed a significant difference between pre- and post-test in plyometrics training group with p = 0,001. Conclusion: Plyometrics training may improve short-term memory and mood in Diponegoro Medical Faculty Students.