Introduction: Sleeping disturbance is commonly found in community especially medical students. The are several factors that may cause sleep disturbance including unrelax prior to sleep. Stimulus Autonomous Sensory Meridian Response (ASMR) is known to having a relax sensation. However there is no report yet about ASMR effect on sleep quality. The aim of this study is to determine the effect of ASMR on medical students sleep quality. Methods: Thirty subjects from undergraduate students of Medical Faculty Diponegoro University academic year 2017 were collected. Written informed consents were collected before recruitment. They were randomly divided into treatment group was given ASMR stimulation (n=15) and control group was given non ASMR video (n=15). ASMR stimulation was delivered by watching a 20 minutes of ASMR video at the night for 14 consecutive days. The subjects were asked to fill out the Pittsburgh Sleep Quality Index (PSQI) questionnaire at before and after intervention. Results: PSQI score in treatment group was significantly reduced after ASMR stimulation (7.0 ± 1.69 vs 3.9 ± 0.9; p=0.001). PSQI score before and after intervention on control group was not significantly different (6.7 ± 1.45 vs 6.8 ± 1.61; p=0.6). PSQI score after of treatment group was significantly lower than control group (p<0.001). PSQI score category after intervention of subjects in treatment group mostly were “good” (73.3% vs 0.0%; p<0.01). Conclusion: ASMR stimulation can improve sleep quality in medical students.