Introduction: Incision wounds occupy the third position (23.2%) of all types of injuries. Wound healing treatment which using chemicals has had a negative impact in the process of wound healing, and therefore the utilization of natural ingredients that contain anti-inflammatory and antioxidant increasingly being used by the public. The purpose of this study was to prove the effect of Apium graveolens Linn to incision wound healing particularly on fibroblast and collagen density. Methods: This research is a randomized post test with control group design which used 20 rats and divided randomly into four groups, which were given treatment for 7 days. Control group received cream without Apium graveolens Linn, positive control group received antibiotic gentamicin cream 0.1%, while the intervention group received various doses (50 and 70%) of Apium graveolens Linn topically. Fibroblast and collagen density areas were assessed microscopically by 2 anatomical pathologists. Data were analyzed and processed using the Oneway ANOVA - Post Hoc Bonferroni test. Results: There were significant differences in the fibroblast density area between the Apium graveolens Linn extract 50% group (p=0.022), and Apium graveolens Linn extract 70% (p=0.019%) with the control group, respectively. There was a significant difference in collagen density between Apium graveolens Linn 50% extract group (p=0.023) and Apium graveolens Linn extract 70% (p=0,000) with the control group, respectively. Conclusion: Apium graveolens Linn extract can accelerate the healing process of incision wounds by increasing fibroblast and collagen density areas.