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Effects of Stichopus hermanni gelatin and local hydroxyapatite on osteoblast number and the viability of fibroblasts
Endang Wahyuningtyas1, Erwan Sugiatno2.
Introduction: Alveolar bone resorption has significant influence on retention and stabilization of dentures. Hy- droxyapatite is a major component of teeth and has a high bone density. Gelatin is commonly used as a bone graft substitute and has amino acid. Stichopus hermanni gelatin extract contains 74% gelatin. The aim of this research was to determine the effects of S. hermanni gelatin and local hydroxyapatite as bone graft substitute on osteoblasts and the viability of fibroblasts. Methods: The subjects were 3 months old male of Sprague Dawley rats were divided into 3 groups. The subjects were decapitated 3, 7, 14, 21, and 28 days after treatment. The bone defect was made on the femur condyle of Sprague Dawley rats. The defect area in the condyle femur was taken and made histology slides with Hematoxilin Eosine staining. A Trinoculer microscope was used to quantify the number of osteoblasts. The two-way ANOVA test was used to analyzed of the data. A toxicity test was performed using the MTT assay. The one way Anova was used to analyzed it. Results: The number of osteoblasts were significant difference between the
S. hermanni gelatin local hydroxyapatite, S. hermanni gelatin, and gelatin groups after 3, 7, 10, 14, and 28 days of treatment (p<0.05). There were significant differences between the MTT absorbances measured in fibroblasts that were treated for 24 h with different concentrations of the S. hermanni gelatin local hydroxyapatite (p<0.05). Conclusion: The S. hermanni gelatin and local hydroxyapatite as bone graft substitute increased osteoblast number compared with S. hermanni gelatin or gelatin alone. Additionally, S. hermanni gelatin and local hydroxyapatite as bone graft substitute did not cause cytotoxicity on fibroblasts.
Affiliation:
- Universitas Gadjah Mada, Indonesia
- Universitas Gadjah Mada, Indonesia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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3 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
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