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Doxycycline incorporated in gelatin - carbonate apatite bone graft material: in vitro evaluation of osteoblastic alkaline phosphatase and porphyromonas gingivalis colonies
Osa Amila Hafiyyah1, Sri Pramestri Lastianny2, Suryono3.
Introduction: Periodontitis treatment should promote the bone regeneration under minimal infection risks during the healing. The incorporation of local antibiotic doxycycline into bone graft material could prevent the bacterial attach- ment which could lead to failure in the regeneration process, but could decline the alkaline phosphatase (ALP) level. Incorporation of doxycycline into carbonate hydroxy apatite–gelatin bone graft should not affect the effectiveness of the antibacterial as well as regenerative properties. The antibacterial effect on Porphyromonas gingivalis and the alkaline phosphatase (ALP) was be evaluated. This study aimed to evaluate the profile of P. gingivalis colony growth and ALP from osteoblast cultures after contact with a doxycycline–carbonate hydroxyapatite–gelatin bone graft. Methods: Dry specimens of 10 mg alloplastic carbonate apatite were embedded with (0.05 %, 0.1 %, and 0.15 %) doxycycline solution for 24 h. In 96-well plates, the specimens were embedded into colonies of P. gingivalis, and then they were moved into a new well of colonies every 24 h. The concentration of colonies was evaluated using a microplate reader. The other specimens were immersed in osteoblastic MCT3T3 culture cells, and then the osteo- blast ALP was evaluated. Results: The concentration of P. gingivalis colonies was inversely proportional to the doxy- cycline concentration. The ALP concentrations showed no statistical difference between groups. Conclusion: These findings demonstrated that doxycycline was incorporated into the gelatin–carbonate apatite material and showed an antibacterial effect up to 72 h but does not alter the effect of ALP in the osteoblast.
Affiliation:
- Universitas Gadjah Mada, Indonesia
- Universitas Gadjah Mada, Indonesia
- Universitas Gadjah Mada, Indonesia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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3 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
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