This study is an attempt to address the relationship between characteristic of trainees, the training design and the working environment and the transfer of training. 211 respondents from different positions in the Malaysian Royal Customs Department of Johore who have attended a customs course took part in the survey. A questionnaire was used as the main data collection method. Spearmen Rank Correlation analysis was used to find direction and strength of correlation between each dependent and independent variables. The result analysis showed that there are significant relationship between the transfer of training and all the independent variable mentioned. Characteristics of the trainees, that is personality, ability and motivation have a direct relationship with the transfer of training From the aspect of the training design, the contents of the training, learning principles and arrangement of learning activities, there is a direct and significant relationship with the training of transfer. Working environment, that is organizational support, information communication technology (ICT) and work place facilities, and opportunities to apply knowledge, skill and ability (KSA) gained from the training have direct and significant relationship with the transfer of training. The findings of the research showed that majority of respondents displayed average personality characteristics. The majority of the respondents were also of the opinion that the training design and the work environment are at average level.