Roselle (Hibiscus sabdariffa L.) is well-known for antioxidant properties and being utilised worldwide in the production of many processed products. Thus, the industrial production increases with the growing demand of roselle-based products. However, there is an alarming issue where the by-products or waste generated from the processing caused numerous risks for humans, animals and the environment. In this case, the utilization of bio-active compounds isolated from waste not only could reduce the risks and the costs for treatment of waste but also could potentially add more value for agricultural and food productions. Hence, this study aims to determine the effect of different concentrations of roselle by-product on physico-chemical content of Japanese quail eggs. Japanese quails were supplemented with roselle by-product to determine the effectiveness of the dietary treatments on the quality of the eggs. Parameters conducted included the number and weight of eggs, colour, pH, Haugh unit, ascorbic acid analysis and DPPH assay. There was no effect on the production viz total eggs and weight but significant differences were in other quality parameters such as albumen redness; albumen yellowness, pH and Haugh unit. Better performance of antioxidant properties can be obtained from treatment one with 2% of roselle by-product as compared to control and other treatments. In conclusion, the roselle by-product has tendency to provide nutritional attributes by antioxidant properties when it is incorporated into Japanese quail egg.