Introduction: Atopic dermatitis is a skin ailment caused by a reaction of immunoglobulin E. One of the factors that could cause complications with this condition is oxidative stress. Pearl Grass is an herb that has been used as a traditional medicine believed to be able to cure many ailments, such as allergies. This study aims to discover the malondialdehyde (MDA) levels in blood serum on mice atopic dermatitis that is treated with pearl grass extract cream. Methods: This study was done during September-November 2018. This study uses an experimental method with completely randomized design. 35 mice blood samples were used and divided to 7 treatment groups. To calcu- late MDA levels in blood a Thiobarbituric Reactive Substance (TBARS) method is used. The MDA levels data gained was then analyzed with an ANOVA test using SPSS 21.0. Results: The result shows that there is a decrease in MDA blood levels on every treatment groups but no significant difference between the malondialdehyde level of atopic dermatitis mice in each group. The malondialdehyde level were obtained ranged from 0.79 nmol/ml to 1.22 nmol/ ml. Conclusion: The MDA level in blood serum of atopic dermatitis mice is not affected by treatments with pearl grass extract cream (Sig>0,05).