Introduction: : Photodynamic Inactivation is a method for inactivating bacteria using a combination of light and photosensitizer (PS) with the appropriate spectrum. This study aims to determine the energy density to reduce Methicillin Resistent Staphylococcus aureus (MRSA) bacteria with the addition of turmeric extract as a PS. Methods: The samples were divided into 3 groups, namely T0 untreated control group, T1 laser diode treatment group, T2 laser diode with curcumin extract treatment group. Treatment uses a 403 nm diode laser with a biofilm life of 48 hours. Samples was treated with variation of irradiation time of laser 30 s, 60 s, 90 s, 120 s and 150 s. Results: The results of the absorbance test showed that the uptake of turmeric extract on the 403 nm diode laser was 95%. The results of the treatments showed that the 403 nm diode laser irradiation with an energy density of 13.56 J/cm2 with the addition of turmeric extract to the MRSA biofilm resulted in a reduction percentage of 83.93% of the biofilm, greater than the 403 nm diode laser treatment with an energy density of 10.17 J/cm2 without turmeric extract of 81.67%. So the addition of PS extract of turmeric increases the effectiveness of photoinactivation of biofilm bacteria that are resistant to antibiotics.