Introduction: Infectious disease is still a major health problem in Indonesia. One of its bacteriological pathogens is Staphylococcus aureus. Morel berry (Physalis angulata L.) is a traditional herb that can be utilized as an antimicrobial agent because of its chemical substances such as flavanoid, alkaloid, tannins, and polyphenol. Purpose: The aim from this study is to determine the antibacterial activity of ethanolic extract of morel berry (Physalis angulata L). Methods: This study used the diffusion method and concentration of 50%, 70%, and 90%. The obtained data were analyzed by One-Way ANOVA. Phytochemical screening disclosed the presence of alkaloids, polyphenol, flavonoids, and tannins in ethanolic extracts. Sole extract of morel berry (Physalis angulata L.) at a concentration of 90% showed the largest inhibition zone as much as 17 mm to Staphylococcus aureus ATCC 25923. Conclusion: The ethanol extracts prove to be potentially effective as natural alternative preventive to fight against Staphylococcus aureus ATCC 25923.