Introduction: One of the origins of hematopoietic stem cells for the treatment of leukemia, myelomas, lymphomas, genetic disorders, immune system defects and blood cell disorders is the donation of umbilical cord blood (UCB). This study is conducted to determine the level of knowledge among Health Science students about UCB donation and to compare the level of knowledge among students from different gender and programs. Methods: A cross-sec- tional study was conducted among health sciences students at a public university in Malaysia. The sample size was calculated by using Raosoft online sample size calculation. A total of 341 students were needed to participate in this study. The questionnaire was distributed randomly to the students using online survey and they were required to respond to a structured questionnaire consisting of 15 items related to UCB donation. Their knowledge was assessed using descriptive statistics. One-way ANOVA test was used to compare the level of knowledge among students from different gender and programs. Results: Out of 341 health sciences students participated in the study, 87.7% were female, with age ranging from 19 to 25 years. The students had a mean knowledge score of 4.78 (SD=2.84). There was a significant difference of knowledge regarding UCB donation between the types of the programs, but no sig- nificant difference in knowledge on UCB between genders. Conclusion: This study revealed that the health science students had insufficient knowledge regarding UCB donation. Efforts should be made to increase the awareness and knowledge of UCB donation among young generations in Malaysia.