Introduction: This paper aimed to examine the efficacy of Carica papaya seed extract (PSE) as the oral toxin com- ponent in two attractive toxic sugar bait (ATSB) formulations on the mortality of female Aedes aegypti and Aedes albopictus in a laboratory setting. Methods: Toxicity bioassays were conducted to determine the PSE concentration needed in ATSB formulations by exposing female Ae. aegypti and Ae. albopictus to seven different PSE concentra- tions for 72 hours. Probit analysis, conducted at 24-hour intervals, provided the median lethal concentration (LC) required to kill 50% and 90% of both mosquito populations; LC50 and LC90, respectively. In two separate cages, both populations of Ae. aegypti and Ae. albopictus female mosquitoes were exposed to ATSB A and ATSB B for 72 hours to determine the mortality rate. Results: The efficacy of 1% PSE was measured by comparing the mortality rates of ATSB A and ATSB B against a control population. After 72 hours of exposure, the results indicated that the Ae. ae- gypti mortality rate was significantly higher in ATSB A (82.50±3.2%) and ATSB B (80.00±2.0%) but there was no significant difference between Ae. albopictus mortality rates in ATSB A (90.00±2.0%) and the control group. Howev- er, the Ae. albopictus mortality rate was found to be significantly higher in ATSB B (96.25±2.4%). Conclusion: The study concluded that, the addition of 1% PSE increase mortality rates in both Aedes species, especially in the Ae. albopictus population.