Introduction: Nicotine administration can cause changes to the aqueous humour resistance, which could influence the intraocular pressure (IOP). This preliminary study is designed to determine the difference and association of IOP in a sample of young adult smokers and non-smokers in Selangor. Methods: Healthy young adult males between 18 and 35 years old who are non-alcoholic and without ocular and systemic diseases were recruited in this study. Smokers were classified into light smokers, moderate smokers, and heavy smokers based on the smoking index. The IOP was measured using Goldmann applanation tonometer on each eye .Results: The analysis involved a total of 34 young adult males (mean age: 23.29 ± 2.19 years), with 17 (50%) smokers and 17 (50%) non-smokers. For smok- ers, the median IOP value was statistically higher (16.00 (1.8) mmHg), than non-smokers (11.70 (1.0) mmHg) (p < 0.001). All smokers were found to be light smokers; light smokers SI 0-50 and light smokers SI 51-100 showed no significant difference on IOP. Spearman’s correlation indicates a moderate positive correlation between the IOP of both eyes to the smoking index, with rs= 0.46 (p = 0.006) Conclusion: This study exemplified a higher IOP among smokers and moderate positive association of smoking index to the IOP among healthy young adults, even for those with a brief history of smoking.