Introduction: Infertility is not only caused due to female reproductive disorders. Several studies shows both husband and wife can be responsible for infertility. Infertility can also be due to idiopathic causes. The Agency for Health Research and Healthcare Quality in Ronchester, Minnesota recommended that anxiety management deals with re- laxation therapies such as music and nature sounds. The purpose of this study was to determine the effect of classical Ayurvedic music therapy on anxiety levels in primary infertility mothers at Medan Johor Puskesmas in 2020. Meth- ods: This research is a quasi-experimental study design and used a pre-test post-test nonequivalent group. The re- search subjects consisted of 20 primary infertility mothers in the Medan Johor Puskesmasin 2020. Results: The results showed that the average (mean)± standard deviation (SD) score before Ayurvedic therapy was 26.9500±2.48098 and after ayurvedic classical music therapy the average decreased to 17.9000±2.38195, this result showed a decrease in the mean value after giving ayurvedic classical music therapy. The results of statistical tests with paired t-test for samples in one group (paired sample test) showed the value of p=0.000 (p<0.05) and t count = 11.768 > t table 1.734 which proved that there was a significant difference between before and after giving Ayurvedic therapy on the level of anxiety in mothers with primary infertility. Conclusion: Present study shows that there is an effect of Ayurvedic music therapy on anxiety levels in primary infertile mothers in the Medan Johor Health Center 2020.