Introduction: Labour pain is a physiological condition generally experienced by almost all women who give birth. Pain causes tachycardia in the mothers, increased oxygen consumption, lactic acid production (lactic), hyperventi- lation with the risk of respiratory alkalosis, and increased skeletal muscle tension. Counter Pressure Regio Sacralis is an effective therapy to reduce labour pain, especially in the first stage. Based on the previous research, Counter-Pres- sure Regio Sacralis therapy, in addition to reducing the pain scale, was also proven to reduce prostaglandin levels (PGE-2) in slightly insignificant amount. Changes in PGE-2 are also suspected of having an impact on the stimulant hormone, the hormone Interleukin-6 (IL-6). This study aimed to see Regio Sacralis Counter Pressure therapy’s effect on IL-6 and prostaglandin levels at the first stage of labour. Methods: The research method used a Quasi Experiment with the inclusion criteria for mothers of stage 1 labour. There was no pelvic neglect and other abnormalities that hindered vaginal, primipara, single pregnancy, where the head was not receiving painkillers. Results: The results showed 25 respondents with an average age of 23.72 (±2.89) years old, the mean IL-6 concentration before therapy was 185.159 (±3.76) pg/ml and after therapy was 180.782 (±22.05) pg/ml, the mean PGE2 concentration before therapy was 223.521 (±72.73) pg/ml and after therapy was 179.873 (± 110.61) pg/ml. Conclusion: Regio Sacralis counter-pressure therapy did not significantly reduce IL-6 and prostaglandin hormone levels in mothers of the first stage labour.