Introduction: Hepatitis B (HBV) infection rate dropped after Universal immunization program for infant launched. However, those persons who born before the program implemented may get HBV at a young age and susceptible to be infected. The present work aims to evaluate the epidemiology of HBV among the students of SMAN Kupang, East Nusa Tenggara. Methods: Total serum samples from 341 subjects, (ages 13-22, mean 17.5 ± 1.69 years; male / female 133/208) have been tested for Surface antigen (HBsAg) and Surface Antibody (anti-HBs) and anti-core antigen (anti-HBc) for HBV. Results: HBsAg, anti-HBc, anti-HBs prevalence has been found 13.2%, 39.8%, 28.7%, respec- tively. It has been concluded that the percentage of young adults who are still vulnerable and at risk of contracting HBV is still high (52.7%). Conclusion: This study emphasizes the relevance of increasing the prevention strategy to filter out and to handle HBV carriers, including the implementation of screening for Hepatitis B and vaccine boosting that have been aimed at young adults.