Antinuclear antibodies (ANA) indirect immunofluorescence assay (IFA) is the first line immunological investigation, mainly for systemic autoimmune rheumatic disease (SARD). However, ANA is also being requested in certain derma- tological conditions particularly in those with immune dysregulation as underlying pathogenesis. It is not uncommon to get positive ANA results in patients with non-SARD dermatological conditions. However, the clinical significance and usefulness of ANA has not been well established. This review summarises the available studies on ANA IFA in non-SARD dermatological conditions which include atopic dermatitis (AD), psoriasis, vitiligo and autoimmune bul- lous diseases. It aims to determine the prevalence of ANA, the commonly reported ANA titres and patterns, as well as any clinical associations between ANA and non-SARD dermatological conditions.