Introduction: Inactive students with poor sleep quality are frequently linked to diminished cognitive function. This study is to evaluate the prevalence of physical activity and sleep quality and to find their relationship with academic achievement among preclinical students in University of Cyberjaya. Methods: A cross sectional study was conducted among pre-clinical MBBS students in University of Cyberjaya, aged 18-30 years and students of at least one semester. Respondents were chosen using convenient sampling. Data were collected using validated online questionnaires. Results: 42.6% of the respondents were physically inactive and 60% of them were less likely to score  3.0 GPA. Physical inactivity was reported the highest among male (45.2%), age group ï‚£ 20 years old (24.6%), Year 2 (30.6%) and Chinese/Indian/Others (75%). Among the physically inactive respondents, the most significant barriers reported were ‘Busy’ (35%), ‘Lazy/Unmotivated’ (27.5%) and ‘Afraid to go out due to COVID-19’ (17.5%). In addition, overall prevalence for poor sleep quality was 23.4% whereas 30% of them were less likely to score  3.0 GPA. High prevalence of poor sleep quality was reported among females (27%), age group  20 years old (31%), Year 2 (24.5%) and Malay (25.7%). Multivariate analysis indicates that there was no association between physical activity and sleep quality with academic achievement (p>0.05). Conclusion: Only minority of preclinical medical students are physically inactive and have poor sleep quality. Both physical activity and sleep quality are also found to have no effect on academic achievement.