Introduction: There are some important factors in preparing a nurse to perform their role as a clinical instructor (CIs) in the clinical setting, including the competence of teaching, organizational support, resources, program planning, teaching-service integration, and student characteristic. Therefore, these factors influence CIs to maintain their role in teaching nursing students in the clinical field. However, there is no study has been found related to that issue. This study aimed to determine the perceptions of CIs towards educational preparation and organizational support in nurs- ing clinical education. Methods: The study design was a cross-sectional. The sampling technique was convenience sampling. 104 respondents were recruited within two weeks. The instruments used are questionnaire about sociode- mographic and preceptor’s perception of their role from another research. Descriptive and correlative analysis using Chi-square were conducted to determine the relationship between demographic factors and CIs perceptions of their roles. Results: The results showed that most CIs had a positive perception of their role. Furthermore, it was known that the length of experience working as CIs was associated with pedagogical competence (p = 0.047), support and educational resources (p = 0.046), and teaching service integration (p = 0.013). Meanwhile, preceptor training was associated with educational program planning (p = 0.037). Conclusion: It can be concluded that although nurses have a positive perception of their role as CIs, several demographic factors influence their competence in teaching. Academic institutions need to facilitate each CIs hospital in gaining teaching competence abilities.