In present time, it is common for any monitoring system to incorporate Internet of Things (IoT) to provide online real-time data. In this project, smart aquaculture monitoring system was developed by combining Wireless Sensor Network (WSN) and Wi-Fi module. In order to verify the system performance, the smart aquaculture monitoring system was tested at actual site located at Fisheries Research Institute (FRI), Gelang Patah, Johor. Three types of sensors (pH, dissolved oxygen and temperature) were deployed in a shrimp pond to monitor the water quality continuously in real-time. The data collected by this system is further analyzed to understand the system stability for different setup. From measurement performed at the site, optimum working condition of system was successfully identified. The system able to provide reliable and accurate data when compared to commercial device used by FRI. Implementation of a smart online monitoring system reduces the use of manpower for monitoring and ensures the water quality is maintained at the acceptable level.