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Antioxidative and photoprotective effects of pleurotus flabellatus (Pink Oyster Mushroom) polysaccharides against UVA Radiation-induced cytotoxicity in human dermal fibroblast (HS-27) cell line
Norintan Suhaida Abdul Rahman1, Nur Izyanni Hamid2, Najwa Mohamad3, Mahani Mahadi4, Ismail Rozila5, Masriana Hassan6, Nur Fatin Zalikha Zailan7, Shaiful Azuar Mohamad8.
Introduction: Ultraviolet (UV) A is the longest wavelength of UV radiation, accounts for approximately 95% of the radiation reaching the earth's surface. It can penetrate deeply into the skin layer and able to induce photoaging and photocarcinogenesis through the activation of reactive oxygen species (ROS). Polysaccharides-containing Pleurotus flabellatus (known as a pink oyster mushroom) has antioxidative properties and may inhibit free radical activities generated from UV radiation. Hence, this present study was to evaluate the antioxidative and photoprotective prop- erties of exopolysaccharides (ExPFE) and exopolysaccharides (EnPFE) of Pleurotus flabellatus extracts on UVA irradi- ated human dermal fibroblast (HS-27) cell line. Methods: The antioxidant level of ExPFE and EnPFE was determined using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, while both cytotoxicity and photoprotective effects of the extracts on the HS-27 cell line were determined using CellTiter-Blue® cell viability assay. The effects of ExPFE and EnPFE on the HS-27 cell migration was evaluated using the scratch assay. Results: Both ExPFE and EnPFE exhib- ited respectable antioxidant and scavenging activity in DPPH. The extracts also demonstrated a non-cytotoxicity, but photoprotective effects to the HS-27 cells by increasing the percentage of cell viability and enhancing cell migration activity upon UVA exposure. Conclusion: The ExPFE and EnPFE exhibit antioxidative and photoprotective effects on UVA irradiated HS-27 cell line. This study suggests that pink oyster polysaccharides could be a potential natural bioactive compound for skin protection against UVA radiation.
Affiliation:
- University of Cyberjaya, Malaysia
- University of Cyberjaya, Malaysia
- University of Cyberjaya, Malaysia
- University of Cyberjaya, Malaysia
- University of Cyberjaya, Malaysia
- Universiti Putra Malaysia (UPM), Malaysia
- Universiti Putra Malaysia (UPM), Malaysia
- Malaysian Nuclear Agency, Malaysia
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Indexation |
Indexed by |
MyJurnal (2021) |
H-Index
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3 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus 2020 |
Impact Factor
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CiteScore (0.2) |
Rank |
Q4 (Medicine (all)) |
Additional Information |
SJR (0.144) |
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