Introduction: Tooth extraction treatment causes damage to the mucosa and surrounding tissue. The body will repair the tissue after tooth extraction by forming collagen fibers. Tempuyung (Sonchus arvensis) leaves contain flavonoids that can help the formation of collagen fibers. This study was to determine the effect of giving tempuyung leaf extract on collagen formation after tooth extraction in rats. Methods: Male rats were extracted from the lower-left incisor, then divided into a control group without extract, and the treatment group was given tempuyung leaf extract at a dose of 100 mg/kg body weight orally once a day. The control group and the treatment group were decapitated on the 7th and 14th days after tooth extraction. Histological samples were stained with Masson’s Trichrome and observed using a digital microscope with a magnification of 40x. Results: The Mann-Whitney test showed that the collagen density in the treatment group was significantly higher than the control group on the 7th and 14th days after the extraction. Conclusion: Tempuyung (Sonchus arvensis) leaf extract effectively increased collagen density in the wound healing process after tooth extraction of rats.