Introduction: Deep bite is a condition that indicates an excess of overbite. Overbite has a normal value of 1/3 of the clinical crown of the lower incisors covered by the upper incisors. The increasing prevalence of deep bite in the mixed dentition are due to the change of deciduous teeth into permanent teeth. Deep bite case that is not resolved immediately can cause more severe malocclusion problems in the permanent dentition, such as soft tissue trauma, tooth wear, damaged periodontal tissues, tooth loss and mobility, and abnormalities in TMJ. This research focus is determining the relationship between skeletal factors and overbite depth in the mixed dentition using Steiner analy- sis. Methods: This study used a cross-sectional study design with an analytical approach. The research sample used a study model and lateral cephalometric radiograph of patients at Rumah Sakit Universitas Brawijaya, Department of Orthodontics. The sample exclusion criteria were damaged sample, sample showing an open bite image, and cepha- lometric radiograph with severe craniofacial damage. There were 57 samples. Measurement of overbite is done using calipers. Cephalometric measurements were performed using Steiner analysis of the skeletal angle of SNA, SNB, and SNGoGn. Results: The data analysis used descriptive statistics and the Spearman correlation test with confidence level up to 95%. The Spearman test angle is carried out by connecting the skeletal angle to the overbite depth. The re- sults were: SNA (r= -0,116 p=0,391), SNB (r=-0,154 p=0,253), and SNGoGn (r=0,034 p=0,802). Conclusion: There is no significant relationship between skeletal factors and overbite depth in the mixed dentition.