Introduction: Macrophage is a phagocytic mononuclear cell that releases IL-1β, a proinflammatory cytokine that is released as a host defense response to injury. Physalis angulata fruit extract gel contains active compounds which one is lupeol, has anti-inflammatory ability by inhibiting the synthesis of proinflammatory cytokines. Objective: To determine the comparison of the administration of Triamcinolone acetonide with Physalis angulata fruit extract gel to the number of macrophages and cellular expression of IL-1β in labial mucous traumatic ulcers wound healing of white rats. Methods: This study was divided into 9 groups with 3 time series (days -2, -6 and -12), and divided into 3 treatment groups (control, physalis angulata fruit extract gel, triamcinolone acetonide), which were induced by trau- matic ulcers. The preparation and staining use HE and IHC. Observation of preparations using a digital microscope. Macrophage and IL-1β counts are calculated using OLYVIA and ImageJ. Results: Data analysis using independent t-test, showed significant differences in the cellular expression of IL-1β between physalis and triamcinolone groups on days 2, 6 and 12. Pearson correlation test showed that the longer treatment in 3 time series (2nd, 6th and 12th) that was carried out could reduce the number of macrophages and IL-1β and the relationship between the number of macrophages and IL-1β in all groups. Conclusion: There is a difference between the administration of triamcino- lone acetonide with physalis angulata fruit extract gel to the cellular expression of interleukin 1 beta (IL-1β) in labial mucous traumatic ulcers wound healing of white rats (Rattus norvegicus).