Introduction: Chronic Periodontitis is simply defined as an infectious disease which affects inflammation in tooth-sup- porting tissues, progressive attachment loss and bone loss. Honey can be an antibacterial agent. It is due to the facts that honey has high sugar content, relatively acidic pH, and low protein content. Thus, honey can limit the avail- able water quantity to inhibit bacterial growth. This research is to determine the ability of the western-bee honey solution in handling the Porphyromonas gingivalis bacteria with agar dilution method. Methods: This research was conducted using laboratory experimental approach, the posttest only control group design which based on in vitro study. The concentrations of the solution used are 5%, 10%, 15% and 20%. The dependent variable in this study is the growth of Porphyromonas gingivalis colony after being administered some western-bee (Apis mellifera) honey solution in varied concentrations. The assessment was based on the thickness of the bacterial colony growth. Results: Based on Kruskal-Wallis test, the result showed that there were significant differences in colony growth between the control and treatment group. The Spearman correlation test resulted that the increasing of solution concentration will decrease the growth of Porphyromonas gingivalis bacteria colony. Conclusion: Based on this research, it can be concluded that the use of western-bee (Apis mellifera) honey solution against Porphyromonas gingivalis bacteria is effective.